Integrity workflow

Yield workflow

Automation

 

Sample integrity and user safety

 

When working with low copy samples, it is essential to minimise the number of times that the sample tube is opened and to minimise the surfaces to which the sample is exposed. These factors are crucial for forensic samples and microbial samples where extraneous bacterial DNA is ubiquitous in the environment.

 

A second advantage to closed-tube methods comes when handling pathogens or human bodily fluids. Any step that has the potential to produce aerosols should be avoided.

 

The simple, one-step, closed-tube ZyGEM method protects your sample and protects you.


 

Yield loss

 

With every step in a DNA-extraction procedure is the potential for yield loss. Yield may not be critical with large samples, for for low-copy samples and trace samples, an advantage of the ZyGEM approach is that there are no need for yield-lowering purification steps. All ZyGEM reagents are compatible with Taq DNA polymerase and so the method is as simple as sample-in, extract-out.

 

 

 

 

 

 

 

 

 

Simplicity

 

Simplicity is crucial for two reasons... there are fewer chances for making mistakes, and simple methods can be automated on off-the-shelf liquid handling robots. Bead based methods and silica column methods can be automated, but only on dedicated equipment. The ZyGEM methods work well with devices that can be used for other processes in your laboratory.

 

See for example: 

 

Automation of saliva FTA extraction on Eppendorf EpMotion (PDF 248k)